Uniconazoletriazole nemai kula da girman shukawanda ake amfani da shi sosai don daidaita tsayin shuka da hana girman tsiron shuka. Duk da haka, tsarin kwayoyin halitta wanda uniconazole ke hana tsawaitar shukar hypocotyl har yanzu ba a fayyace shi ba, kuma akwai wasu bincike kaɗan da suka haɗa bayanan transcriptome da metabolome don bincika hanyar tsawaitar hypocotyl. A nan, mun lura cewa uniconazole ya hana tsawaitar hypocotyl sosai a cikin shukar kabeji masu fure na kasar Sin. Abin sha'awa, bisa ga haɗin nazarin transcriptome da metabolome, mun gano cewa uniconazole ya shafi hanyar "phenylpropanoid biosynthesis". A cikin wannan hanyar, kwayar halitta guda ɗaya kawai ta dangin enzyme regulatory gene, BrPAL4, wacce ke da hannu a cikin biosynthesis na lignin, an rage ta sosai. Bugu da ƙari, gwaje-gwajen yisti ɗaya-haɗi da biyu-haɗi sun nuna cewa BrbZIP39 zai iya ɗaure kai tsaye zuwa yankin mai haɓaka BrPAL4 kuma ya kunna fassararsa. Tsarin dakatar da kwayoyin halitta da kwayar cutar ta haifar ya ƙara tabbatar da cewa BrbZIP39 na iya daidaita tsawaitar kabejin China da kuma haɗakar lignin hypocotyl. Sakamakon wannan binciken ya samar da sabbin bayanai game da tsarin sarrafa kwayoyin halitta na cloconazole wajen hana tsawaitar kabejin China. An tabbatar da cewa cloconazole ya rage yawan lignin ta hanyar hana haɗakar phenylpropanoid da BrbZIP39-BrPAL4 ke jagoranta, wanda hakan ya haifar da raguwar hypocotyl a cikin shukar kabejin China.
Kabeji na kasar Sin (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) na cikin dangin Brassica kuma sanannen kayan lambu ne na shekara-shekara da ake nomawa a kasarmu (Wang et al., 2022; Yue et al., 2022). A cikin 'yan shekarun nan, girman samar da farin kabeji na kasar Sin ya ci gaba da fadada, kuma hanyar noma ta canza daga shuka kai tsaye ta gargajiya zuwa shukar da aka yi da kuma dasawa sosai. Duk da haka, a cikin tsarin shukar da aka yi da kuma dasawa mai tsanani, girman hypocotyl da ya wuce kima yana haifar da samar da 'ya'yan itatuwa masu kafafu, wanda ke haifar da rashin ingancin shuka. Saboda haka, sarrafa girman hypocotyl da ya wuce kima matsala ce mai matukar muhimmanci a cikin al'adun shukar da kuma dasa kabeji na kasar Sin. A halin yanzu, akwai wasu bincike da suka hada da bayanan transcriptomics da metabolomics don bincika tsarin tsawaita hypocotyl. Ba a yi nazarin tsarin kwayoyin halitta da chlorantazole ke sarrafa fadada hypocotyl a cikin kabeji na kasar Sin ba tukuna. Mun yi niyyar gano waɗanne kwayoyin halitta da hanyoyin kwayoyin halitta ke amsawa ga hypocotyl da uniconazole ke haifarwa a cikin kabeji na kasar Sin. Ta amfani da nazarin transcriptome da metabolomic, da kuma nazarin yeast one-hybrid, gwajin luciferase dual, da gwajin hana kwayoyin halitta (VIGS), mun gano cewa uniconazole na iya haifar da hypocotyl dwarfing a cikin kabejin China ta hanyar hana lignin biosynthesis a cikin shukar kabejin China. Sakamakonmu yana ba da sabbin fahimta game da tsarin sarrafa kwayoyin halitta wanda uniconazole ke hana tsawaita hypocotyl a cikin kabejin China ta hanyar hana haɓakar biosynthesis na phenylpropanoid wanda tsarin BrbZIP39–BrPAL4 ke jagoranta. Waɗannan sakamakon na iya samun mahimman tasirin aiki don inganta ingancin shukar kasuwanci da kuma ba da gudummawa ga tabbatar da yawan amfanin gona da ingancin kayan lambu.
An saka cikakken BrbZIP39 ORF a cikin pGreenll 62-SK don samar da mai tasiri, kuma an haɗa ɓangaren mai haɓaka BrPAL4 zuwa kwayar halittar mai rahoto ta pGreenll 0800 luciferase (LUC) don samar da kwayar halittar mai rahoto. An haɗa ƙwayoyin halittar mai tasiri da mai rahoto zuwa ganyen taba (Nicotiana benthamiana).
Domin fayyace alaƙar da ke tsakanin metabolites da kwayoyin halitta, mun yi wani bincike na haɗin gwiwa da kuma na transcriptome. Binciken haɓaka hanyoyin KEGG ya nuna cewa DEGs da DAMs sun haɗu a cikin hanyoyin KEGG guda 33 (Hoto na 5A). Daga cikinsu, hanyar "phenylpropanoid biosynthesis" ita ce mafi wadata; hanyar "photosynthesis carbon fixation", hanyar "flavonoid biosynthesis", hanyar "pentose-glucuronic acid interconversion", hanyar "tryptophan metabolism", da kuma hanyar "starch-sucrose metabolism" suma an wadatar da su sosai. Taswirar tattara zafi (Hoto na 5B) ya nuna cewa DAMs da ke da alaƙa da DEGs an raba su zuwa rukuni da dama, daga cikinsu flavonoids sune mafi girman rukuni, yana nuna cewa hanyar "phenylpropanoid biosynthesis" ta taka muhimmiyar rawa a cikin hypocotyl dwarfism.
Marubutan sun bayyana cewa an gudanar da binciken ne ba tare da wata alaƙa ta kasuwanci ko ta kuɗi ba wadda za a iya fassara ta a matsayin wata matsala ta sha'awa.
Duk ra'ayoyin da aka bayyana a cikin wannan labarin na marubucin ne kawai kuma ba lallai bane ya nuna ra'ayoyin ƙungiyoyi masu alaƙa, masu bugawa, editoci, ko masu bita ba. Duk wani samfura da aka tantance a cikin wannan labarin ko ikirarin da masana'antun su suka yi ba shi da garantin ko amincewa daga mai wallafawa.
Lokacin Saƙo: Maris-24-2025