Uniconazoletriazole nemai sarrafa girma shukawanda ake amfani da shi sosai don daidaita tsayin shuka da kuma hana ci gaban seedling. Duk da haka, tsarin kwayoyin halitta wanda uniconazole ke hana seedling hypocotyl elongation har yanzu ba a sani ba, kuma akwai wasu 'yan nazarin da suka haɗu da bayanan da aka rubuta da kuma metabolome don bincika tsarin haɓakar hypocotyl. A nan, mun lura da cewa uniconazole muhimmanci hana hypocotyl elongation a kasar Sin flowering kabeji seedlings. Abin sha'awa, dangane da haɗaɗɗen fassarar fassarar da metabolome, mun gano cewa uniconazole yana tasiri sosai akan hanyar "phenylpropanoid biosynthesis". A cikin wannan hanyar, ƙwayar cuta guda ɗaya tak na dangin tsarin halittar enzyme, BrPAL4, wanda ke da hannu a cikin biosynthesis na lignin, ya ragu sosai. Bugu da kari, yisti-wani matasan-hybrid da 'yansayen kafaffun kafa biyu sun nuna cewa brbzip39 zai iya taka rawa kai tsaye ga yankin mai gabatarwa na BRPAL4 kuma yana kunna rubutun ta. Tsarin shiru na kwayar cutar da ke haifar da kwayar cutar ya kara tabbatar da cewa BrbZIP39 na iya daidaita tasirin hypocotyl na kabeji na kasar Sin da hadewar hypocotyl lignin. Sakamakon wannan binciken ya ba da sababbin fahimta game da tsarin tsarin kwayoyin halitta na cloconazole a cikin hana haɓakar hypocotyl na kabeji na kasar Sin. An tabbatar a karon farko cewa cloconazole ya rage abun ciki na lignin ta hanyar hana haɗin phenylpropanoid wanda ke yin sulhu ta hanyar BrbZIP39-BrPAL4, wanda hakan ya haifar da dwarfing hypocotyl a cikin tsire-tsire na kabeji na kasar Sin.
Kabeji na kasar Sin (Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee) na dangin Brassica ne kuma sanannen kayan lambu ne na cruciferous na shekara-shekara wanda ake girma a cikin ƙasata (Wang et al., 2022; Yue et al., 2022). A cikin 'yan shekarun baya-bayan nan, yawan noman farin kabeji na kasar Sin ya ci gaba da habaka, kuma tsarin noman ya canza daga yadda ake yin shuka kai tsaye zuwa ga al'adun dasa shuki. Koyaya, a cikin aiwatar da al'adun shuka mai zurfi da dasawa, haɓakar hypocotyl da yawa yana ƙoƙarin samar da tsire-tsire masu tsire-tsire, yana haifar da ƙarancin ingancin seedling. Don haka, sarrafa girman girman hypocotyl da ya wuce kima wani lamari ne mai mahimmanci a cikin al'adun shuka mai zurfi da dashen kabeji na kasar Sin. A halin yanzu, akwai ƴan karatun da ke haɗa bayanan rubutu da bayanan metabolomics don gano tsarin haɓakar hypocotyl. Har yanzu ba a yi nazarin tsarin kwayoyin halittar da chlorantazole ke sarrafa yaduwar hypocotyl a cikin kabeji na kasar Sin ba. Mun yi nufin gano wane nau'in kwayoyin halitta da hanyoyin kwayoyin ke amsawa ga dwarfing hypocotyl da ke haifar da uniconazole a cikin kabeji na kasar Sin. Yin amfani da nazarin bayanan rubutu da metabolomic, da yisti-hybrid analysis, dual luciferase assay, da ƙwayoyin cuta-induced gene silencing (VIGS), mun gano cewa uniconazole na iya haifar da hypocotyl dwarfing a cikin Sin kabeji ta hana lignin biosynthesis a cikin Sin kabeji shuka. Sakamakonmu yana ba da sabon haske game da tsarin tsarin kwayoyin halitta wanda uniconazole ke hana haɓakar hypocotyl a cikin kabeji na Sinanci ta hanyar hana phenylpropanoid biosynthesis wanda tsarin BrbZIP39-BrPAL4 ya shiga tsakani. Waɗannan sakamakon na iya samun mahimman abubuwan aiki don haɓaka ingancin shukar kasuwanci da ba da gudummawa don tabbatar da yawan amfanin ƙasa da ingancin kayan lambu.
An saka BrbZIP39 ORF mai cikakken tsayi a cikin pGreenll 62-SK don samar da mai tasiri, kuma an haɗa guntun mai talla na BrPAL4 zuwa ga pGreenll 0800 luciferase (LUC) mai ba da rahoto don samar da jigilar mai rahoto. An canza masu tasiri da masu ba da rahoto zuwa ga ganyen taba (Nicotiana benthamiana).
Don fayyace alaƙar metabolites da kwayoyin halitta, mun yi nazarin metabolome na haɗin gwiwa da nazarin kwafi. Binciken haɓaka hanyoyin KEGG ya nuna cewa DEGs da DAMs an haɓaka su tare a cikin hanyoyin 33 KEGG (Hoto 5A). Daga cikin su, hanyar "phenylpropanoid biosynthesis" ita ce mafi mahimmancin wadata; Hanyar “photosynthetic carbon fixation”, hanyar “flavonoid biosynthesis”, hanyar “pentose-glucuronic acid interconversion”, hanyar “tryptophan metabolism”, da hanyar “sitaci-sucrose metabolism” suma sun sami wadata sosai. Taswirar tarin zafi (Hoto 5B) ya nuna cewa DAMs masu alaƙa da DEGs sun kasu kashi da yawa, daga cikinsu flavonoids sune mafi girman nau'in, yana nuna cewa hanyar "phenylpropanoid biosynthesis" ta taka muhimmiyar rawa a cikin dwarfism hypocotyl.
Marubutan sun bayyana cewa an gudanar da binciken ne ba tare da wata alaƙar kasuwanci ko ta kuɗi da za a iya fassara ta a matsayin rikici mai ban sha'awa ba.
Duk ra'ayoyin da aka bayyana a cikin wannan labarin na marubucin ne kawai kuma ba lallai ba ne su yi daidai da ra'ayoyin ƙungiyoyi masu alaƙa, masu bugawa, editoci, ko masu bita. Duk wani samfur da aka kimanta a cikin wannan labarin ko iƙirarin da masana'antunsu suka yi ba su da garantin ko mawallafin ya amince da su.
Lokacin aikawa: Maris 24-2025