Paclobutrazol yana haifar da biosynthesis na triterpenoid ta hanyar danne mai sarrafa rubutu mara kyau SlMYB a cikin honeysuckle na Japan.

Manyan namomin kaza suna da tarin abubuwa masu aiki iri-iri kuma ana ɗaukar su a matsayin albarkatun halittu masu mahimmanci. Phellinus ignarius babban namomin kaza ne da aka saba amfani da shi don dalilai na magani da abinci, amma rarrabuwarsa da sunan Latin har yanzu suna da ce-ce-ku-ce. Ta amfani da nazarin daidaiton sassan kwayoyin halitta da yawa, masu binciken sun tabbatar da cewa Phellinus ignarius da wasu nau'ikan halittu suna cikin sabon nau'in kuma sun kafa halittar Sanghuangporus. Namomin kaza na honeysuckle Sanghuangporus lonicericola yana ɗaya daga cikin nau'ikan Sanghuangporus da aka gano a duk duniya. Phellinus ignarius ya jawo hankali sosai saboda nau'ikan kaddarorin magani daban-daban, gami da polysaccharides, polyphenols, terpenes, da flavonoids. Triterpenes sune manyan mahaɗan wannan nau'in, suna nuna ayyukan antioxidant, antibacterial, da antitumor.
Triterpenoids suna da babban damar amfani da su a kasuwanci. Saboda ƙarancin albarkatun Sanghuangporus na daji a cikin yanayi, haɓaka ingancinsa da yawan amfanin sa yana da matuƙar muhimmanci. A halin yanzu, an sami ci gaba wajen haɓaka samar da nau'ikan metabolites na biyu na Sanghuangporus ta hanyar amfani da sinadarai masu haifar da sinadarai don sarrafa dabarun fermentation a cikin ruwa. Misali, an nuna cewa fatty acids masu yawan polyunsaturated, fungal elicators11 da phytohormones (gami da methyl jasmonate da salicylic acid14) suna ƙara yawan samar da triterpenoid a Sanghuangporus. Masu kula da haɓakar shuka(PGRs)zai iya daidaita tsarin biosynthesis na metabolites na biyu a cikin tsire-tsire. A cikin wannan binciken, an binciki PBZ, wani mai kula da ci gaban shuka wanda ake amfani da shi sosai don daidaita ci gaban shuka, yawan amfanin ƙasa, inganci da halayen jiki. Musamman ma, amfani da PBZ na iya yin tasiri ga hanyar biosynthetic ta terpenoid a cikin tsire-tsire. Haɗa gibberellins tare da PBZ ya ƙara yawan quinone methide triterpene (QT) a cikin Montevidia floribunda. An canza tsarin hanyar terpenoid na man lavender bayan an yi masa magani da 400 ppm PBZ. Duk da haka, babu rahotanni kan amfani da PBZ ga namomin kaza.
Baya ga binciken da aka mayar da hankali kan karuwar samar da triterpene, wasu bincike sun kuma bayyana hanyoyin da ake bi wajen daidaita biosynthesis na triterpene a cikin Moriformis karkashin tasirin sinadarai masu haifar da sinadarai. A halin yanzu, bincike yana mai da hankali kan sauya matakan bayyanar kwayoyin halitta masu alaƙa da biosynthesis na triterpene a cikin hanyar MVA, wanda ke haifar da karuwar samar da terpenoid.12,14 Duk da haka, hanyoyin da ke ƙarƙashin waɗannan kwayoyin halitta masu tsari da aka sani, musamman abubuwan da ke daidaita bayyanar su, ba su da tabbas a cikin hanyoyin da ake bi wajen daidaita biosynthesis na triterpene a cikin Moriformis.
A cikin wannan binciken, an binciki tasirin yawan masu kula da girmar tsirrai (PGRs) daban-daban akan samar da triterpene da kuma girman mycelium yayin fermentation na honeysuckle (S. lonicericola) a cikin ruwa. Daga baya, an yi amfani da metabolomics da transcriptomics don nazarin tsarin triterpene da tsarin bayyanar kwayoyin halitta da ke cikin biosynthesis na triterpene yayin maganin PBZ. Bayanan jerin RNA da bioinformatics sun kara gano ma'aunin rubutun da aka yi niyya na MYB (SlMYB). Bugu da ƙari, an samar da maye gurbi don tabbatar da tasirin tsari na kwayar halittar SlMYB akan biosynthesis na triterpene da kuma gano kwayoyin halittar da za a iya niyya. An yi amfani da gwaje-gwajen canjin motsi na electrophoretic (EMSA) don tabbatar da hulɗar furotin na SlMYB tare da masu haɓaka kwayoyin halittar SlMYB. A taƙaice, manufar wannan binciken ita ce a ƙarfafa biosynthesis na triterpene ta amfani da PBZ da kuma gano wani abu mai suna MYB transcription factor (SlMYB) wanda ke daidaita kwayoyin halittar biosynthetic na triterpene kai tsaye ciki har da MVD, IDI, da FDPS a cikin S. lonicericola a matsayin martani ga shigar PBZ.
Haɗakar IAA da PBZ ya ƙara yawan samar da triterpenoid a cikin honeysuckle, amma tasirin haɗakar PBZ ya fi bayyana. Saboda haka, an gano cewa PBZ shine mafi kyawun mai haifar da ƙarin yawan 100 mg/L, wanda ya cancanci ƙarin bincike.