Manya-manyan namomin kaza suna da wadataccen tsari iri-iri na metabolites masu rai kuma ana ɗaukar su albarkatun halittu masu mahimmanci. Phellinus igniarius babban naman kaza ne da aka saba amfani da shi don magani da dalilai na abinci, amma rabe-rabensa da sunan Latin suna ci gaba da jayayya. Yin amfani da nazarin daidaitawar sassan multigene, masu binciken sun tabbatar da cewa Phellinus igniarius da nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'in nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i nau'i). Namomin kaza Sanghuangporus lonicericola ɗaya ne daga cikin nau'in Sanghuangporus da aka gano a duk duniya. Phellinus igniarius ya jawo hankali sosai saboda nau'ikan magunguna daban-daban, ciki har da polysaccharides, polyphenols, terpenes, da flavonoids. Triterpenes su ne mabuɗin magungunan magunguna na wannan nau'in, suna nuna ayyukan antioxidant, antibacterial, da antitumor.
Triterpenoids suna da babban yuwuwar aikace-aikacen kasuwanci. Saboda ƙarancin albarkatun Sanghuangporus na daji a cikin yanayi, haɓaka ingantaccen aikin sa na biosynthetic da yawan amfanin ƙasa yana da mahimmanci. A halin yanzu, an sami ci gaba wajen haɓaka samar da ƙwayoyin metabolites daban-daban na Sannghuangporus ta hanyar amfani da inducers na sinadarai don sarrafa dabarun fermentation a cikin ruwa. Misali, polyunsaturated fatty acids, fungal elicitors11 da phytohormones (ciki har da methyl jasmonate da salicylic acid14) an nuna su don haɓaka samar da triterpenoid a cikin Sanghuangporus. Masu kula da girma shuka(PGRs)na iya daidaita biosynthesis na metabolites na biyu a cikin tsire-tsire. A cikin wannan binciken, an bincika PBZ, mai kula da haɓakar tsire-tsire da aka yi amfani da shi sosai don daidaita haɓakar shuka, yawan amfanin ƙasa, inganci da halayen ilimin lissafi. Musamman, amfani da PBZ na iya rinjayar hanyar terpenoid biosynthetic a cikin tsire-tsire. Haɗuwa da gibberellins tare da PBZ ya ƙãra abun ciki na quinone methide triterpene (QT) a cikin Montevidia floribunda. An canza abun da ke cikin hanyar terpenoid na man lavender bayan jiyya tare da 400 ppm PBZ. Koyaya, babu rahotanni game da aikace-aikacen PBZ zuwa namomin kaza.
Bugu da ƙari, nazarin da ke mayar da hankali kan karuwa a cikin samar da triterpene, wasu nazarin sun kuma bayyana hanyoyin da aka tsara na triterpene biosynthesis a cikin Moriformis a ƙarƙashin rinjayar masu haifar da sinadarai. A halin yanzu, nazarin yana mai da hankali kan sauya matakan maganganu na kwayoyin halitta masu alaka da triterpene biosynthesis a cikin hanyar MVA, wanda ke haifar da karuwa a cikin samar da terpenoid.12,14 Duk da haka, hanyoyin da ke ƙarƙashin waɗannan sanannun kwayoyin halitta, musamman ma abubuwan da aka rubuta da ke tsara maganganun su, sun kasance ba a sani ba a cikin tsarin tsarin biosynthesis na triterpe.
A cikin wannan binciken, an binciko tasirin nau'ikan nau'ikan nau'ikan haɓakar shuka (PGRs) akan samar da triterpene da haɓakar mycelial yayin fermentation na honeysuckle (S. lonicericola). Bayan haka, an yi amfani da metabolomics da transcriptomics don nazarin abubuwan da ke tattare da triterpene da tsarin maganganun kwayoyin halitta da ke cikin biosynthesis na triterpene yayin jiyya na PBZ. RNA-sequencing da bayanan bioinformatics sun kara gano maƙasudin rubutun MYB (SlMYB). Bugu da ƙari, an ƙirƙira mutants don tabbatar da tasiri na tsarin SlMYB akan biosynthesis na triterpene da kuma gano yuwuwar kwayoyin halitta. An yi amfani da gwaje-gwajen motsi na motsi na lantarki (EMSA) don tabbatar da hulɗar furotin SlMYB tare da masu tallata kwayoyin SlMYB. A taƙaice, makasudin wannan binciken shine don tayar da biosynthesis na triterpene ta amfani da PBZ da kuma gano wani nau'in rubutun MYB (SlMYB) wanda ke daidaita kwayoyin halittun biosynthetic triterpene kai tsaye ciki har da MVD, IDI, da FDPS a cikin S. lonicericola don amsawa ga shigar da PBZ.
Gabatar da duka IAA da PBZ sun ƙara haɓaka samar da triterpenoid a cikin honeysuckle, amma tasirin shigar da PBZ ya fi bayyana. Sabili da haka, an gano PBZ a matsayin mafi kyawun inducer a ƙarin ƙaddamarwa na 100 mg / L, wanda ya cancanci ƙarin nazarin.
Lokacin aikawa: Agusta-19-2025