Rarraba ruwan sama na yanayi na yanayi a lardin Guizhou bai yi daidai ba, tare da yawan hazo a bazara da bazara, amma ciyawar da aka yi wa fyaden tana iya fuskantar matsalar fari a cikin kaka da hunturu, wanda ke matukar shafar amfanin gona. Mustard shuka ce ta musamman da ake nomawa a lardin Guizhou. Yana da ƙarfin jurewar fari kuma ana iya shuka shi a cikin wuraren tsaunuka. Yana da wadataccen albarkatu na kwayoyin halitta masu jure fari. Gano kwayoyin halitta masu jure fari yana da matukar muhimmanci ga inganta irin mustard. da sabbin abubuwa a albarkatun germplasm. Iyalin GRF suna taka muhimmiyar rawa wajen haɓaka tsiro da haɓakawa da martani ga damuwa na fari. A halin yanzu, an gano kwayoyin GRF a Arabidopsis 2, shinkafa (Oryza sativa) 12, rapeseed 13, auduga (Gossypium hirsutum) 14, alkama (Triticum). aestivum) 15, gero lu'u-lu'u (Setaria italica) 16 da Brassica17, amma babu rahoton kwayoyin GRF da aka gano a cikin mustard. A cikin wannan binciken, an gano kwayoyin halittar dangin GRF na mustard a matakin genome-fadi da halayensu na zahiri da sinadarai, alaƙar juyin halitta, homology, abubuwan da aka kiyaye, tsarin kwayoyin halitta, kwafin kwayoyin halitta, cis-elements da matakin seedling (matakin ganye huɗu). An yi nazarin sifofin maganganun da ke ƙarƙashin damuwa na fari don samar da tushen kimiyya don ƙarin nazarin kan yuwuwar aikin ƙwayoyin halittar BjGRF a cikin martanin fari da kuma samar da jinsin ɗan takara don kiwo mustard mai jure fari.
An gano kwayoyin halittar BjGRF 34 a cikin Brassica juncea genome ta amfani da binciken HMMER guda biyu, dukkansu sun ƙunshi yankunan QLQ da WRC. An gabatar da jerin CDS na ƙwayoyin halittar BjGRF da aka gano a cikin Ƙarin Tebura S1. BjGRF01-BjGRF34 ana kiran su bisa ga wurinsu akan chromosome. Abubuwan sinadarai na physicochemical na wannan iyali suna nuna cewa tsawon amino acid yana da matukar canzawa, daga 261 aa (BjGRF19) zuwa 905 aa (BjGRF28). Matsayin isoelectric na BjGRF ya tashi daga 6.19 (BjGRF02) zuwa 9.35 (BjGRF03) tare da matsakaicin 8.33, kuma 88.24% na BjGRF shine furotin na asali. Matsakaicin nauyin kwayoyin da aka annabta na BjGRF yana daga 29.82 kDa (BjGRF19) zuwa 102.90 kDa (BjGRF28); ma'aunin rashin kwanciyar hankali na sunadaran BjGRF ya fito daga 51.13 (BjGRF08) zuwa 78.24 (BjGRF19), duk sun fi 40, yana nuna cewa ma'aunin fatty acid ya fito daga 43.65 (BjGRF01) zuwa 78.78 (BjGRF08) (BjGRF08) (BjGRF19) daga matsakaicin hydrophilic (RA) (BjGRF31) zuwa -0.45 (BjGRF22), duk sunadaran BjGRF na hydrophilic suna da ƙimar GRAVY mara kyau, wanda zai iya zama saboda rashin ƙarancin hydrophobicity wanda ragowar ya haifar. Hasashen ƙananan ƙwayoyin cuta ya nuna cewa 31 BjGRF sunadaran sunadaran za a iya zama a cikin tsakiya a cikin tsakiya, BjGRF04 za a iya zama a cikin peroxisomes, BjGRF25 za a iya zama a cikin cytoplasm, kuma BjGRF28 za a iya zama a cikin chloroplasts (Table 1), yana nuna cewa BjGRF na iya zama muhimmiyar rawa a cikin tsakiya. factor transcription.
Binciken Phylogenetic na dangi a cikin jinsuna daban daban na iya taimakawa wajen nazarin ayyukan kwayoyin halitta. Saboda haka, an zazzage cikakken jerin amino acid na 35 na rapeseed, turnip 16, shinkafa 12, gero 10 da 9 Arabidopsis GRFs kuma an gina bishiyar phylogenetic bisa 34 da aka gano BjGRF genes (Fig. 1). Iyalai ukun sun ƙunshi lambobi daban-daban na membobi; 116 GRF TFs sun kasu gida uku daban-daban (ƙungiyoyi A ~ C), sun ƙunshi 59 (50.86%), 34 (29.31%) da 23 (19.83)% na GRFs, bi da bi. Daga cikin su, 'yan uwa na BjGRF 34 sun warwatse a cikin iyalai 3: Membobi 13 a rukunin A (38.24%), mambobi 12 a rukunin B (35.29%) da mambobi 9 a rukunin C (26.47%). A cikin aiwatar da polyploidization na mustard, adadin kwayoyin halittar BjGRFs a cikin dangi daban-daban ya bambanta, kuma haɓakawa da hasara na iya faruwa. Ya kamata a lura da cewa ba a rarraba shinkafa da gero a rukunin C, yayin da akwai GRF guda 2 da GRF guda 1 a rukunin B, kuma galibin shinkafa da gero GRFs an hada su ne a reshe daya, wanda ke nuni da cewa BjGRFs na da alaka da dicots. Daga cikin su, mafi zurfin bincike akan aikin GRF a cikin Arabidopsis thaliana yana ba da tushe don nazarin aikin BjGRFs.
Phylogenetic bishiyar mustard ciki har da Brassica napus, Brassica napus, shinkafa, gero da membobin dangin Arabidopsis thaliana GRF.
Binciken kwayoyin halitta masu maimaitawa a cikin dangin GRF mustard. Layin launin toka a bango yana wakiltar shingen aiki tare a cikin kwayoyin halittar mustard, layin ja yana wakiltar nau'i-nau'i na maimaitawa na BjGRF;
Maganar kwayar halittar BjGRF a ƙarƙashin damuwa na fari a matakin ganye na huɗu. Ana nuna bayanan qRT-PCR a cikin Ƙarin Tebura S5. Babban bambance-bambance a cikin bayanai ana nuna su ta ƙananan haruffa.
Yayin da yanayin duniya ke ci gaba da canzawa, nazarin yadda amfanin gona ke jure wa matsalar fari da inganta hanyoyin jurewa ya zama wani babban batu na bincike18. Bayan fari, tsarin ilimin halittar jiki, maganganun kwayoyin halitta da hanyoyin rayuwa na shuke-shuke za su canza, wanda zai iya haifar da dakatar da photosynthesis da damuwa na rayuwa, yana shafar yawan amfanin gona da ingancin amfanin gona19,20,21. Lokacin da tsire-tsire ke jin alamun fari, suna samar da manzanni na biyu kamar Ca2 + da phosphatidylinositol, suna ƙara haɓakar ƙwayar calcium ion na ciki kuma suna kunna hanyar sadarwa ta hanyar hanyar phosphorylation protein22,23. Ƙarshen furotin da aka yi niyya na ƙarshe yana da hannu kai tsaye a cikin tsaro ta salula ko kuma yana daidaita maganganun kwayoyin halitta masu dangantaka ta hanyar TFs, inganta haɓakar tsire-tsire zuwa damuwa24,25. Don haka, TFs suna taka muhimmiyar rawa wajen magance matsalolin fari. Dangane da jeri da kaddarorin daurin DNA na TFs na damuwa na fari, ana iya raba TFs zuwa iyalai daban-daban, kamar GRF, ERF, MYB, WRKY da sauran iyalai26.
Iyalin jinsin GRF wani nau'in TF ne na musamman na shuka wanda ke taka muhimmiyar rawa a fannoni daban-daban kamar girma, haɓakawa, watsa sigina da martanin kariyar shuka27. Tun lokacin da aka gano Grf na farko a O. Sativa28, an bayyana ƙari da yawa a cikin yawancin jinsuna da kuma amsawa da damuwa, 29, 30,31,32. Tare da buga jerin kwayoyin halittar Brassica juncea, gano dangin BjGRF ya zama mai yiwuwa33. A cikin wannan binciken, an gano kwayoyin halittar BjGRF 34 a cikin dukkanin kwayoyin halittar mustard kuma suna BjGRF01-BjGRF34 dangane da matsayinsu na chromosomal. Dukkansu sun ƙunshi manyan wuraren QLQ da WRC da aka kiyaye su. Binciken kaddarorin sunadarai na physicochemical ya nuna cewa bambance-bambance a cikin lambobin amino acid da ma'aunin kwayoyin halitta na sunadaran BjGRF (sai BjGRF28) ba su da mahimmanci, yana nuna cewa dangin BjGRF na iya samun ayyuka iri ɗaya. Binciken tsarin halitta ya nuna cewa 64.7% na kwayoyin BjGRF sun ƙunshi exons 4, yana nuna cewa tsarin halittar BjGRF yana da ɗan kiyaye shi a cikin juyin halitta, amma adadin exons a cikin BjGRF10, BjGRF16, BjGRP28 da BjGRF29 ya fi girma. Nazarin ya nuna cewa ƙari ko share exons ko introns na iya haifar da bambance-bambance a cikin tsarin kwayoyin halitta da aiki, ta haka ne ya haifar da sababbin kwayoyin halitta34,35,36. Saboda haka, muna tsammanin cewa intron na BjGRF ya ɓace a lokacin juyin halitta, wanda zai iya haifar da canje-canje a aikin kwayoyin halitta. Daidai da binciken da ake ciki, mun kuma gano cewa adadin introns yana da alaƙa da maganganun kwayoyin halitta. Lokacin da adadin introns a cikin kwayar halitta ya yi girma, kwayar halitta na iya amsawa da sauri ga wasu abubuwa marasa kyau.
Kwafin Halittu babban abu ne a cikin juyin halittar kwayoyin halitta da kwayoyin halitta37. Nazarin da ke da alaƙa sun nuna cewa kwafin kwayoyin halitta ba kawai yana ƙara yawan ƙwayoyin GRF ba, har ma yana aiki a matsayin hanyar samar da sababbin kwayoyin halitta don taimakawa tsire-tsire su dace da yanayin muhalli daban-daban38. An sami jimillar nau'ikan nau'ikan kwayoyin halitta guda 48 a cikin wannan binciken, dukkansu kwafi ne na kashi, wanda ke nuni da cewa kwafin sassan su ne babbar hanyar kara yawan kwayoyin halitta a cikin wannan iyali. An ba da rahoto a cikin wallafe-wallafen cewa kwafi na yanki zai iya inganta haɓakar dangin GRF a cikin Arabidopsis da strawberry, kuma ba a sami kwafi na wannan dangin a cikin kowane nau'in ba27,39. Sakamakon wannan binciken ya yi daidai da binciken da aka yi a kan Arabidopsis thaliana da iyalan strawberry, yana nuna cewa dangin GRF na iya ƙara yawan kwayoyin halitta da kuma samar da sababbin kwayoyin halitta ta hanyar kwafi a cikin nau'i-nau'i daban-daban.
A cikin wannan binciken, an gano jimillar kwayoyin halittar BjGRF 34 a cikin mustard, waɗanda aka raba zuwa 3 subfamilies. Waɗannan kwayoyin halitta sun nuna irin wannan tsare-tsare da tsarin halittar. Binciken Collinearity ya nuna nau'i-nau'i 48 na kwafi a cikin mustard. Yankin mai gabatarwa BjGRF ya ƙunshi abubuwan cis-aiki da ke da alaƙa da amsa haske, amsawar hormonal, amsa damuwa na muhalli, da haɓakawa da haɓakawa. An gano bayanin kwayoyin halittar BjGRF 34 a matakin shuka mustard (tushen, mai tushe, ganyaye), da kuma yanayin yanayin 10 BjGRF a ƙarƙashin yanayin fari. An gano cewa alamun bayyanar cututtuka na BjGRF a ƙarƙashin damuwa na fari sun kasance iri ɗaya kuma yana iya zama kama. shiga cikin tsarin tilasta tilasta fari. Ƙwayoyin BjGRF03 da BjGRF32 na iya taka rawa mai kyau na tsari a cikin damuwa na fari, yayin da BjGRF06 da BjGRF23 ke taka rawa a cikin damuwa na fari a matsayin miR396 kwayoyin manufa. Gabaɗaya, bincikenmu yana ba da tushen nazarin halittu don gano aikin ƙwayar halittar BjGRF a nan gaba a cikin tsire-tsire na Brassicaceae.
Cibiyoyin mastad da aka yi amfani da su a cikin wannan gwaji an samar da su ne daga Cibiyar Binciken iri ta Guizhou, Kwalejin Kimiyyar Noma ta Guizhou. Zaɓi dukan tsaba kuma dasa su a cikin ƙasa (substrate: ƙasa = 3: 1), kuma tattara tushen, mai tushe da ganye bayan mataki na ganye hudu. An bi da tsire-tsire tare da 20% PEG 6000 don kwatanta fari, kuma an tattara ganyen bayan 0, 3, 6, 12 da 24 hours. An daskare duk samfuran shuka nan da nan a cikin ruwa nitrogen sannan a adana su a cikin injin daskarewa -80 ° C don gwaji na gaba.
Duk bayanan da aka samu ko aka tantance yayin wannan binciken an haɗa su a cikin labarin da aka buga da fayilolin ƙarin bayanai.
Lokacin aikawa: Janairu-22-2025